| Title: HIV-1 spreads from lymphocytes to normal human
keratinocytes suitable for autologous and allogenic transplantation. |
| Title Abreviation: J Invest Dermatol |
Date of Pub: 1995 Nov |
| Author: Ramarli D; Giri A; Reina S;
Poffe O; Cancedda R; Varnier O; Tridente G; De Luca M; |
| Issue/Part/Supplement: 5 |
Volume Issue: 105 |
Pagination: 644-7 |
| MESH Headings: Acquired Immunodeficiency Syndrome
(*TM); Cell Line; Cell Transplantation; Cell-Free System; Human; HIV Core
Protein p24 (ME); HIV-1 (*/PH); |
| Journal Title Code: IHZ |
Publication Type: JOURNAL ARTICLE |
| Date of Entry: 951208N |
Entry Month: 9602 |
| Country: UNITED STATES |
Index Priority: 1 |
| Language: Eng |
Unique Identifier: 96067276 |
| Unique Identifier: 96067276 |
ISSN: 0022-202X |
| Abstract: Normal human keratinocytes can reconstitute
in vitro cohesive sheets of epithelium suitable for grafting onto patients.
Despite the widespread use of autografts and allografts, no data are yet
available on productive infection by human immunodeficiency virus (HIV-1)
of human keratinocytes. To address this point, we challenged keratinocytes
at the second passage of culture with HTLV-IIIB virus by cell-free and
cell-mediated inoculum. Viral entry was not achieved by cell-free inoculum,
thus demonstrating that cultured keratinocytes do not provide the membrane
requirements for viral binding and/or internalization. By contrast, the
cell-mediated inoculum overcame specific receptor constraints, leading
to viral integration and productive infection. The p24gag viral protein
was transiently released in the culture supernatant, although at low level.
The viral progeny produced by infected keratinocytes was rescued and amplified
by co-culture experiments performed with the HIV-1 high sensitive CEM-SS
human T-cell line. Viral integration, p24gag production, and secondary
transmission to lymphoid cells was further confirmed with keratinocytes
infected at the fourth passage of culture. Taken together, our results
demonstrate that cultured keratinocytes can be infected by HTLV-IIIB virus,
which can be maintained in semi-latent form for several passages after
inoculum and rescued to full replication by a proper target. The in vitro
demonstration of lympho-epithelial HIV-1 spreadings warns against the use
of inappropriately screened biopsies for the preparation of skin grafts.
|
| Abstract By: Author |
| Address: Institute of Immunology and Infectious Diseases,
University of Verona, Italy. |
| Title: HTLV type IIIB infection of human thymic epithelial
cells: viral expression correlates with the induction of NF-kappa B-binding
activity in cells activated by cell adhesion. |
| Title Abreviation: AIDS Res Hum Retroviruses |
Date of Pub: 1996 Sep 1 |
| Author: Ramarli D; Reina S; Merola M;
Scupoli MT; Poffe O; Riviera AP; Brentegani M; Fiorini E; Vella A; Varnier
O; Tridente G; |
| Issue/Part/Supplement: 13 |
Volume Issue: 12 |
Pagination: 1217-25 |
| MESH Headings: Cell Adhesion*; Cell Line*; Child,
Preschool*; Epithelium*; Human; HIV-1*; Interleukin-6*; NF-kappa B*; |
| Journal Title Code: ART |
Publication Type: JOURNAL ARTICLE |
| Date of Entry: 970113N |
Entry Month: 9703 |
| Country: UNITED STATES |
Index Priority: 2 |
| Language: Eng |
Unique Identifier: 97024615 |
| Unique Identifier: 97024615 |
ISSN: 0889-2229 |
| Abstract: Productive infection by the LAV strain has
been demonstrated in T cell precursors at different stages of intrathymic
development, while viral replication in thymic epithelial cells is still
controversial. In this article we show that epithelial cell cultures derived
from the medullary component of normal thymus are infectable by HTLV-IIIB
virus through cell-free and lymphoid-mediated transmission. Free virus
inoculum results in the integration of proviral copies undergoing poor
replication, whereas lymphoid-mediated transmission leads to substantial
viral expression and the production of viral progeny able to secondary
infect lymphoid cells. Interleukin 6 production and phenotype changes (increased
expression of MHC class I and ICAM-1) were induced in TE cells by contact
with free virus or by adhesion to infected lymphoid cells. By contrast,
NF-kappa B-binding activity on the HIV-1 LTR kappa B enhancer element was
upregulated only by contact with infected lymphoid cells, but not with
virus. The viral replication observed in TE cells after lymphoid-mediated
transmission correlates with the upregulation of NF-kappa B-binding activity.
Interleukin 6 increased production and phenotype changes and increased
NF-kappa B-binding activity were also induced by adhesion to uninfected
lymphoid cells, demonstrating that lymphoepithelial cell contacts can activate
TE cells. These results demonstrate that thymic epithelial cells are permissive
to HIV infection and that viral replication in this cell lineage can be
modulated by intracellular signals delivered by adhesive contacts. |
| Abstract By: Author |
| Address: Instituto di Immunologia e Malattie Infettive,
UniversitÍa di Verona, Italy. |
| Title: Serological, biological, and molecular characterization
of New Zealand white rabbits infected by intraperitoneal inoculation with
cell-free human immunodeficiency virus. |
| Title Abreviation: J Virol |
Date of Pub: 1993 Sep |
| Author: Reina S; Markham P; Gard E;
Rayed F; Reitz M; Gallo RC; Varnier OE; |
| Issue/Part/Supplement: 9 |
Volume Issue: 67 |
Pagination: 5367-74 |
| MESH Headings: Acquired Immunodeficiency Syndrome
(BL/IM/*PP); Animal; Blotting, Western; Cell Line; Cell-Free System; Disease
Models, Animal; DNA, Viral (BL); Human; HIV Antibodies (*BL); HIV Core
Protein p24 (BL); HIV-1 (*/IP/PH); |
| Journal Title Code: KCV |
Publication Type: JOURNAL ARTICLE |
| Date of Entry: 930913N |
Entry Month: 9311 |
| Country: UNITED STATES |
Index Priority: 2 |
| Language: Eng |
Unique Identifier: 93353613 |
| Unique Identifier: 93353613 |
ISSN: 0022-538X |
| Abstract: The availability of a small laboratory animal
model suitable for the evaluation of methods for prevention and treatment
of human immunodeficiency virus type 1 infection would be a valuable resource
for AIDS research. Here we describe the infection of a strain of domestic
rabbits by intraperitoneal inoculation with cell-free human immunodeficiency
virus type 1. Evidence of infection includes the presence of an immune
response that has persisted for almost 3 years and the detection of an
reisolation of infectious virus from peripheral blood mononuclear cells
(PBMCs) and other tissues during the first 2 years. Typical viral proteins,
DNA and RNA patterns, were observed in rabbit PBMCs and in cells infected
by cocultivation with rabbit PBMCs. While a number of possible pathological
changes were evaluated in infected rabbits, the presence of changes in
lymph node structure similar to those reported in infected humans merits
further investigation. |
| Abstract By: Author |
| Address: Laboratory of Human Retrovirology, School
of Medicine, University of Genoa, Italy. |
| Title: Knowledge data base system for twins study.
|
| Title Abreviation: Acta Genet Med Gemellol (Roma)
|
Date of Pub: 1994 |
| Author: Reina S; Miozza F; |
| Issue/Part/Supplement: 1-2 |
Volume Issue: 43 |
Pagination: 83-8 |
| MESH Headings: Data Collection; Human; Information
Systems (*); Italy (EP); Software; Twin Studies (*SN); Twins (SN); -AA-;
|
| Journal Title Code: 0P6 |
Publication Type: JOURNAL ARTICLE |
| Date of Entry: 950306N |
Entry Month: 9505 |
| Country: ITALY |
Index Priority: 2 |
| Language: Eng |
Unique Identifier: 95149641 |
| Unique Identifier: 95149641 |
ISSN: 0001-5660 |
| Abstract: The medical research on twins, carried out
at the Gregor Mendel Institute for Medical Genetics and Twin Study in Rome
over the past four decades, has resulted in a vast collection of clinical
paper records. A challenge was presented by the need for a more secure
method of storage to preserve this enormously valuable historical and scientific
patrimony and to render its contents more easily accessible for research
purposes. We met the challenge by planning and developing the computerization
of this material. New concepts, currently being explored in biomedical
informatics, were applied to build a Knowledge Data Base System, using
a fourth-generation language (SQL). This architecturally innovative computer
system enables its users to manipulate data supplied, rather than just
simply storing it. Based on heuristic relational criteria between variables
and parameters, the system is employed to solve problems of sibling design
analysis typically arising from twins' records, but is also equipped to
meet future data base requirements. Another feature of the system is its
users' ability to pull off data in the form of regular automated reports,
which are distributed through a Local Area Network (LAN). Through a Bulletin
Board System (BBS) and modem, any scientist (outside as well as within
the Institute) is thus able to access data and exchange scientific information.
|
| Abstract By: Author |
| Address: Informatics Laboratory, Gregor Mendel Institute,
Rome, Italy. |
| Number of References: 25 |
| Title: Genetic recombination by spheroplast fusion
in Escherichia coli K12. |
| Title Abreviation: Cytobios |
Date of Pub: 1993 |
| Author: Reina S; Debbia EA; Schito GC;
|
| Issue/Part/Supplement: 305 |
Volume Issue: 76 |
Pagination: 91-5 |
| MESH Headings: Calcium Chloride (PD); Escherichia
coli (DE/*GE); Gene Deletion; Gene Transfer; Membrane Fusion; Polymyxin
B (PD); Recombination, Genetic (*PH); Spheroplasts (DE/*ME); -RN-; |
| Journal Title Code: DXE |
Publication Type: JOURNAL ARTICLE |
| Date of Entry: 940302N |
Entry Month: 9405 |
| Country: ENGLAND |
Index Priority: 2 |
| Language: Eng |
Unique Identifier: 94123597 |
| Unique Identifier: 94123597 |
ISSN: 0011-4529 |
| Abstract: Genetic transfer mediated by spheroplast
formation and fusion in Escherichia coli was studied. Recombination did
not occur from spheroplasts prepared by the combined glycine and lysozyme-EDTA
treatment described by Coetzee et al. (1979). In contrast, when bacteria
were exposed to a sub-inhibitory concentration of polymyxin B (0.5 microgram/ml)
during the spheroplast generation phase, recombinants arose at frequencies
of 1 x 10(-8) to 2.6 x 10(-8). The incidence of genetic transfer was further
increased by adding 0.01 M CaCl2 to the polyethylene glycol fusion mixture
(from 9 x 10(-7) to 9 x 10(-8). Finally, when the effects of both polymyxin
B and calcium chloride were combined recombinants arose at frequencies
of 3.4 x 10(-6) to 7.3 x 10(-7). These findings suggest that the detergent-like
action of polymyxin B in removing a large part of the outer membrane enhances
plasma membrane availability where fusion can take place. |
| Abstract By: Author |
| Address: Institute of Microbiology, University of
Genoa, Italy. |